Publications

2020 - present

Heller, L., Bulysheva, A., Arpag, S., Sales Conniff, A., Kohena, K., Shi, G., Semenova, N., Heller, R., Cemazar, M., Growth environment influences B16.F10 mouse melanoma cell response to gene electrotransfer, Bioelectrochemistry, 140:107827, 2021. PMID: 33971375.

Dolezalova, E., Malik, M. A., Heller, L., Heller, R., Delivery and expression of plasmid DNA into cells by a novel non-thermal plasma source, Bioelectrochemistry 140:107816, 2021. PMID: 33894566.

Bulysheva, A., Heller, L., Francis, M., Varghese, F., Boye, C., Heller, R., Monopolar Gene Electrotransfer Enhances Plasmid DNA Delivery to Skin, Bioelectrochemistry, 140:107814, 2021.  PMID: 33962133.

Jesenko, T., Bosnjak, M., Markelc, B., Sersa,G, Znidar, K.,  Heller, L., Cemazar, M., Radiation induced upregulation of DNA sensing pathways is cell-type specific and can mediate the off-target effects, Cancers, 12:3365-3388, 2020. PMID: 33202881

Heller, L., Thinard, R., Chevalier, M., Arpag, S., Jing, Y., Greferath, R., Heller, R., Nicolau, C., Secretion of proteins and antibody fragments from transiently transfected endothelial progenitor cells, Journal of Cellular and Molecular Medicine, 24:8772-8778, 2020. PMID: 32610368.

Bošnjak, M., Znidar, K., Tur, J., Sales Conniff, A., Kohena, K., Jesenko, T., Marklec, B., Semenova, N., Heller, L., Cemazar M. In vitro and in vivo correlation of skin and cellular responses to nucleic acid delivery, Biomedicine & Pharmacotherapy 150:113088, 2022. PMID: 35658241.

Sales Conniff A, Encalada G, Patel S, Bhandary M, Al-Takrouri F, Heller L, Poly(I:C) transfection induces a pro-inflammatory cascade in murine mammary carcinoma and fibrosarcoma cells. RNA Biology 19:841, 2022. PMID: 35737804

2010 - 2019

Semenova, N., Bosnjack, M., Markelc, B., Znidar, K., Cemazar, M., Heller, L., Multiple cytosolic DNA sensors bind plasmid DNA after transfection. Nucleic Acids Research, 47:10235–10246, 2019. PMID: 31495892 

Znidar K., Bosnjak M., Semenova N., Pakhomova O., Heller L., Cemazar M. Tumor cell death after electrotransfer of plasmid DNA is associated with cytosolic DNA sensor upregulation, Oncotarget, 9:18665-18681, 2018. PMID: 29721152

Znidar K., Bosnjak M., Jesenko, T., Heller L., Cemazar M., Upregulation of DNA sensors in B16.F10 melanoma spheroid cells after electrotransfer of pDNA. Technol Cancer Res Treat, doi: 10.1177/1533033818780088, 2018. PMID: 29879868

Dutta,S., Celestine, M.J., Khanal, S., Huddleston, A., Simms, C., Arca, J.F., Mitra, A., Heller, L., Kraj, P.J., Ledizet, M., Anderson, J.F., Neelakanta, G., Holder, A.A., Sultana, H. Coordination of different complexes to copper(II) and cobalt(III) metal centers enhances Zika virus and dengue virus loads in both arthropod cells and human keratinocytes, Biochimica and Biophysica Acta, 1862(1): 40-50, 2018. PMID: 29030319

Heller, L.C. and Edelblute, C.E. Long-term metabolic persistence of Gram positive bacteria on healthcare-relevant plastic, American Journal of Infection Control, 46(1): 50-53, 2018. PMID: 28918990

Bosnjak M., Jesenko, T., Kamensek, U., Sersa, G., Lavrencak, J., Heller L., Cemazar M. Electrotransfer of different control plasmids elicits different antitumor effectiveness in B16.F10 melanoma, Cancers, 10(2), pii: E37, 2018. PMID: 29382170

Neuber, J.U., Song, S., Malik, M.A., Heller, L., and Jiang, C. Nanosecond Pulsed Plasma Brush for Bacterial Inactivation on Laminate. IEEE TRPMS, 1(4):368 – 375, 2017.

Muratori, C., Pakhomov, A., Heller, L., Casciola, M., Gianulis, E., Grigoryev, S., Xiao, S., Pakhomova, O. Electrosensitization increases antitumor effectiveness of nanosecond pulsed electric field in vivo, Technol Cancer Res Treat,. doi: 10.1177/1533034617712397, 2017. PMID: 28585492

Edelblute, C.E., Malik, M.A, Heller, L.C. Antibacterial efficacy of a novel plasma reactor without an applied gas flow against methicillin resistant Staphylococcus aureus on diverse surfaces, Bioelectrochemistry, 2016, 112:106-111. PMID: 27095606.

Edelblute, C.E., Heller, L.C., Malik, M.A., Bulysheva, A., Heller, R. Plasma activated air mediates plasmid DNA delivery in vivo, Mol Ther  Methods Clin Dev 3:16028, 2016. PMID: 27110584.

Žnidar, K., Bosnjak, M., Heller, L.C., Cemazar, M. Cytosolic DNA sensor upregulation accompanies DNA electrotransfer in B16.F10 melanoma cells, Mol Ther Nucleic Acids 5(6):e322, 2016. PMID: 27271988

Edelblute, C., Malik, M., Heller, L. Surface-dependent inactivation of model microorganisms with shielded sliding atmospheric plasma discharges and applied air flow, Bioelectrochemistry 103:22-27, 2015. PMID: 25200988.

Bosnjak, M., Dolinsek, T., Cemazar, M., Kranjc, S., Blagus, T., Markelc, B., Stimac, M., Kamensek, U., Heller, L., Bouquet, C., Sersa, G. Gene electrotransfer of Plasmid AMEP, an integrin targeted therapy, has antitumor and antiangiogenic action in murine B16 melanoma. Gene Ther, 22:578-590, 2015. PMID: 25781650.

Edelblute, C.E., Heller, L.C., Malik, M.A., Heller, R. Activated air produced by shielded sliding discharge plasma mediates plasmid DNA delivery to mammalian cells. Biotechnol Bioeng, 112:2583-2590, 2015. PMID:26041378

Edelblute, C.E., Pakhomova, O.N, Li, F. Hargrave, B.Y.,  Heller, L.C. Donor platelet plasma components inactivate sensitive and multidrug resistant Acinetobacter baumannii isolates. Pathog Dis, 73(9). pii: ftv095, 2015. PMID: 26500225

Edelblute, C., Donate, A., Hargrave, B., Heller, L. Human platelet gel supernatant inactivates opportunistic wound pathogens on skin, Platelets 26(1):13-6, 2015. PMID: 24433255

Hao, X., Mattson, A.M., Edelblute, C.E., Malik, M.A., Heller, L.C., Kolb, J.F. Nitric oxide generation with an air-operated non-thermal plasma jet and associated microbial inactivation mechanisms, Plasma Processes and Polymers 11:1044-1056, 2014.

Heller, L.C., Todorovic, Vesna, Cemazar, M. Electrotransfer of single-stranded or double-stranded DNA induces complete regression of palpable B16.F10 mouse melanomas, Cancer Gene Ther 20(12): 695-700, 2013. PMID: 24287723

Heller, L.C., Edelblute, C.M., Mattson, A. M., Hao, X., Singh, T, Kolb, J.K. Inactivation of bacterial opportunistic skin pathogens by nonthermal DC-operated afterglow atmospheric plasma, Letters in Applied Micro 54(2):126-32, 2012. PMID: 22122358

Kolb, J.F., Mattson, A. M., Edelblute, C.M., Hao, X., Price, R.O., Singh, T.J., Malik, M.A., Heller, L. C. Cold DC Operated Air Plasma Jet for the Inactivation of Infectious Microorganisms, IEEE Transactions on Plasma Science, 40(11):3007-3026, 2012.

Guo, S., Donate, A., Basu, G., Lundberg, C., Heller, L., Heller, R. Characterization of gene electrodelivery to skin using a noninvasive multielectrode array, J Control Release 151(3):256-262, 2011. PMID: 21262290

Heller, R., Cruz, Y., Heller, L., Gilbert, R., Jaroszeski, M.  Electrically mediated delivery of plasmid DNA to the skin using a multi-electrode array, Hum Gene Ther 21:357-362, 2010. PMID: 19839722

Marshall, Jr, W. G., Boone, B.A. Burgos, J. D., Gografe, S. I., Baldwin, M. K., Danielson, M. L., Larson, M. J., Caretto, D. R., Cruz, Y., Ferraro, B., Heller, L. C., Ugen, K. E., Jaroszeski, M.J., Heller, R.  Electroporation Mediated Delivery of a Naked DNA Plasmid Expressing VEGF to the Porcine Heart Enhances Protein Expression, Gene Ther 17: 419-423, 2010. PMID: 19956270

Heller, L.C., Heller, R. Electroporation Gene Therapy Preclinical and Clinical Trials for Melanoma, Curr Gene Ther 10:312-7, 2010. PMID: 20557286

Heller, L.C., Cruz, Y. L., Ferraro, B., Yang, H., Heller, R.   Plasmid injection and application of electric pulses alter endogenous mRNA and protein expression in B16.F10 mouse melanomas, Cancer Gene Ther 17(12):864-871, 2010. PMID: 20706286. PMC2981654

Ferraro, B., Heller, L.C., Cruz, Y.L., Guo, S., Donate, A., Heller, R. Evaluation of delivery conditions for cutaneous plasmid electrotransfer using a multielectrode array. Gene Ther 18(5):496-500, 2010. PMID: 21179175

2000 - 2009

Heller, L.C., Jones, M., and Widen, R.H.  Comparison of DNA pyrosequencing to alternative methods for identification of mycobacteria, J Clin Micro 46:2092-2094, 2008. PMID: 18417659

Heller, L.C., Jaroszeski, M.J., Coppola, D., and Heller, R.  Comparison of electrically mediated and liposome-complexed plasmid DNA delivery to the skin, Genet Vaccines Ther 6:16, 2008. PMID: 19055808

Keeling, W.B., Myers, A. R., Stone, P.A., Heller, L., Widen, R., Back, M.R., Johnson, B.L., Bandyk, D.F., Shames, M.L. Regional antibiotic delivery for the treatment of experimental prosthetic graft infections. J Surg Res 157(2):223-6, 2008. PMID: 19560786

Hickey, J.D., Heller, L., Heller, R., and Gilbert, R. Electric field mediated DNA motion model.  Bioelectrochemistry, 70:101-103, 2007. PMID: 16714149

Heller, L.C., McCrae, A.N., Jaroszeski, M.J., Hickey, J., and Heller, R. Optimization of cutaneous electrically mediated plasmid DNA delivery using novel electrode, Gene Therapy 14:275-80, 2007. PMID: 16988718

Moroney, S.M., Heller, L.C., Arbuckle, J., Talavera, M., Widen, R.H.  Staphylococcal Cassette Chromosome mec (SCCmec) and Panton-Valentine Leukocidin Characterization of Methicillin-Resistant Staphylococcus aureus Clones.  J Clin Micro 45:1019-21, 2007. PMID: 17192420

Peak, K.K., Duncan, K.E., Veguilla, W., Luna, V.A., King, D.S., Heller, L., Heberlein-Larson, L., Reeves, F., Cannons, A., Amuso, P., and Cattani, J.  Bacillus acidiceler sp. nov., isolated from forensic specimens, containing Bacillus anthracis pX02 genes, Int JSyst Evol Microbiol, 57: 2031-2036, 2007. PMID: 17766868

Moroney, S., Heller, L., and Widen, R.  Evaluation of two Taqman Real-time PCR assays for the detection of Human Immunodeficiency Virus type I proviral DNA in blood samples, J Micro Methods 65:350-353, 2006. PMID: 16169104

Heller, L. Merkler, K., Westover, J., Cruz, Y., Coppola, D., Benson, K., Daud, A. and Heller, R. Evaluation of toxicity following electrically mediated interleukin-12 gene delivery in a B16 mouse melanoma model, Clin Cancer Res.12:3177-3183, 2006. PMID: 16707618

Luna, V.A., King, D.S., Peak, K.K., Reeves, F., Heberlein-Larson, L., Veguilla, W., Heller, L., Duncan, K.E., Cannons, A.C., Amuso, P., and Cattani, J. Bacillus anthracis virulent plasmid pX02 genes found in large plasmids of two other Bacillus sp. J Clin Micro 44:2367-2377, 2006. PMID: 16825351

Heller, L.C. and Heller, R. In vivo electroporation for gene therapy.  Human Gene Ther 17:890-897, 2006. PMID: 16972757

Heller, L.C., Ugen, K., and Heller, R.  Electroporation for targeted gene transfer, Expert Opin Drug Deliv 2:255-268, 2005. PMID: 16296752

Davis, C.R., Heller, L.C., Peak, K.K., Wingfield, D.L., Goldstein-Hart, C.L., Bodager, D.W., Cannons, A.C., Amuso, P.T., and Cattani, J.  Real time PCR detection of th thermostable direct hemolysin and thermolabile hemolysin genes in a Vibrio parahaemolyticus cultured from mussels associated with a food borne outbreak, J Food Prot 67:1005-1008, 2004. PMID: 15151240

Heller, L., Davis, C., Peak, K., Wingfield, D., Cannons, A., Amuso, P., and Cattini, J.  Comparison of bacterial DNA isolation methods from food samples for detection of shiga toxin producing E. coli by real time PCR, App Env Micro 69:1844-1846, 2003. PMID: 12620880

Heller, L. and Coppola, D. Electrically mediated delivery of vector plasmid DNA elicits an antitumor effect, Gene Ther 9:1321-1325, 2002. PMID: 12224015

Heller, L., Ingram, S., Lucas, L., Gilbert, R., and Heller, R.  Effect of electrically mediated intratumor and intramuscular delivery of a plasmid encoding interferon alpha on visible B16 melanomas, Tech Cancer Res & Treat 1:205-209, 2002. PMID: 12622513

Lucas, M.L., Heller, L., Coppola, D., and Heller, R. IL-12 plasmid delivery by in vivo electroporation for the successful treatment of established subcutaneous B16.F10 melanoma.  Mol Ther 5:668-675, 2002. PMID: 12027550

Gilbert, R., Jaroszeski, M.J., Heller, L., and Heller, R. Electric Field Enhanced Plasmid Delivery to Liver Hepatocellular Carcinomas, Tech Cancer Res & Treat 1:355-363, 2002. PMID: 12625761

Heller, R., Schultz, J., Lucas, M.L., Jaroszeski, M.J., Heller, L.C., Gilbert, R.A., Moelling, K., and Nicolau, C. Intradermal delivery of IL-12 plasmid DNA by in vivo electroporation, DNA and Cell Biol 20:21-26, 2001. PMID: 11242540

Heller, L.C., Jaroszeski, M.J., Coppola, D., Pottinger, C., Gilbert, R., and Heller, R. Electrically mediated plasmid DNA delivery to hepatocellular carcinomas in vivo. Gene Ther 7:826-829, 2000. PMID: 10845719

Heller, L.C., Pottinger, C., Jaroszeski, M.J., Gilbert, R., and Heller, R.  In vivo electroporation of plasmids encoding GMCSF and interleukin-2 into existing B16 melanomas combined with electrochemotherapy induces long term antitumor immunity, Melanoma Res 10:577-583, 2000. PMID: 11198480

Heller, L.C. and Lucas, M.L. Delivery of plasmid DNA by in vivo electroporation.  Gene Ther and Mol Biol 5:50-55, 2000.

Pre - 2000

Heller, L.C., Li, Y., Abrams, K.A. and Rogers, M.  Transcriptional regulation of the Bmp2 gene: Retinoic acid induction in F9 embryonal carcinoma cells and Saccharomyces cerevisiae.  J. Biol. Chem. 274:1394-1400, 1999. PMID: 9880512

Rogers, M.B., Glozak, M.A., and Heller, L.C.  Induction of altered gene expression in early embryos.  Mutation Research 396:79-95, 1997. PMID: 9434861

Heller, L., Orlean, P., and Adair, W.L., Jr.  Saccharomyces cerevisiae sec59 cells are deficient in dolichol kinase activity. Proceedings of the National Academy of Science, USA 89:7013-7016, 1992. PMID: 1323123

Book Chapters

Heller, L., Heller, R. Gene Electrotransfer, “Electroporation in Veterinary Oncology Practice: Electrochemotherapy and Gene Electrotransfer for Immunotherapy”, Joseph A.  Impellizeri, Ed., Springer Nature. 2021. pp. ISBN: 978-3-030-80667-5

Heller, L.C. and Malik, M.A. Microbial Decontamination using Atmospheric Pressure Plasma, “Bioelectrics”, edited by Akiyama, H. and Heller, R., Eds. Springer Publishing, Japan. 2016. pp. 389. Doi: 10.1007/978-4-431-56095-1. ISBN: 978-4-431-56093-7

Heller, L.C. Principles of Electroporation for Gene Therapy, “Handbook of Electroporation”, Miklavcic, D., Ed. Springer Publishing, New York City, NY, USA. 2016. doi: 10.1007/978-3-319-26779-1 48-1. ISBN: 978-3-319-26779-1

Heller, R. and Heller, L.C. Gene Electrotransfer Clinical Trials, “Non-Viral Vectors for Gene Therapy: Physical Methods and Medical Translation (Advances in Genetics)”, Huang, L., Lui, D., and Wagner, E., Eds. Elsevier Inc., San Diego, CA. 2015. pp. 235–262. ISBN: 9780128022726. PMID: 25620013

Shirley, S., Heller, L., Heller, R. Gene Electrotransfer, “Gene and Cell Therapy: Therapeutic Mechanisms and Strategies, Fourth Edition”, Templeton, N.S., Ed., CRC Press, Boca Raton, FL. 2015. ISBN: 9781466571990

Shirley, S.A., Heller, R., and Heller, L.C. Electroporation Gene Therapy, “Gene Therapy of Cancer: Translational Approaches from Preclinical Studies to Clinical Implementation”, Third Edition, Lattime, E.C. and Gerson, S. L., Eds. Elsevier Inc., San Diego, CA. 2013. pp. 93-106. ISBN: 9780123942951

Heller, L. C. and Heller, R.  Gene Electrotransfer to Tumor, “Electroporation in Science and Medicine”, Kee, S., Lee, E., and Gehl, J., Eds., Springer-Verlag, NY, NY. 2011. ISBN: 9781441983626

Heller, L. C. and Heller, R. Translation of electroporation mediated DNA delivery to the clinic, “Advanced Electroporation Techniques in Biology and Medicine”, Markov, M.S., Miklavcic, D. and Pakhomov, A.G., Eds., CRC Press, Boca Raton, FL. 2010. pp. 371-384. ISBN: 9781439819067

Jaroszeski, M.J., Heller, L.C., Gilbert, R., and Heller, R.  Electrically mediated plasmid DNA delivery to solid tumors, “Gene Delivery to Mammalian Cells:  Methods and Protocols”, William C. Heiser, ed., Humana Press, Totowa, NJ. 2004. pp. 237-244. ISBN: 1588290867. PMID: 14707384